Molecular imaging with the PET tracer Zirconium-89 (89Zr, t½ = 78.4 h) can considerably accelerate drug development and facilitate improved patient selection. This has been demonstrated in several pre-clinical and clinical studies. The pharmaceutical industry now realizes that 89Zr-PET can offer excellent sensitivity and accurate quantification, from mouse to man. Some pharmaceutical companies have already shown their interest in 89Zr-immuno-PET, and also academic biomedical research institutions are directed to initiate 89Zr-immuno-PET studies.
To fulfill the needs of potential 89Zr users, BV Cyclotron VU facilitates with our partners on the campus of VU Amsterdam University a dedicated training to help new customers implement 89Zr-radiolabeling at their facilities.
During the 3-day training, the following topics will be practiced and discussed:
- Conjugation of DFO-chelate to a monoclonal antibody (two methods).
- Radiolabeling of conjugated DFO-mAb with 89Zr.
- Purification of radiolabeled 89Zr-DFO-mAb
- Quality control of 89Zr-DFO-mAb.
- GMP aspects to start up clinical 89Zr-immuno-PET studies.
Apart from these, other alternative topics can be discussed in advance and added to the training, whenever possible.
The aim of this program is to train researchers who are interested in immuno-PET with 89Zr, teaching them how to perform the radiolabeling of mAbs and mAb-fragments with 89Zr, and subsequently start pre-clinical and clinical studies at their own location. The training is provided as a 3-day course in Amsterdam. A training at the customer’s location is possible upon request.
See what our trainees say about the training:
“We started the 89Zr labeling course having only a theoretical background in monoclonal antibody modification and 89Zr radiolabeling. During the course we learned in a very clearly explained and totally hands-on manner how to derivatize and subsequently label mAbs with 89Zr. We were also allowed to perform a complete quality control including HPLC analysis, SDS-Page and cell binding assay. The booklet that was provided including all the necessary protocols allowed us to independently reproduce the acquired practical skills on our own mAbs. We highly recommend this course to anyone who would like to radiolabel mAbs or any other molecule with 89Zr.”
For availability and prices, please contact our Product Specialist, Arnold Spaans.
You can reach him at:
Phone: +31 20 4444380